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orai1 (nm_032790) human tagged orf clone  (OriGene)


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    OriGene orai1 (nm_032790) human tagged orf clone
    Orai1 (Nm 032790) Human Tagged Orf Clone, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+orai1+coding+sequence/ORAI1+(NM_032790)+Human+Tagged+ORF+Clone/origene___rc210034
    Average 94 stars, based on 7 article reviews
    orai1 (nm_032790) human tagged orf clone - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Subcloning:

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 (28) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence (NM_032790; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 ( 28 ) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_032790","term_id":"170932551","term_text":"NM_032790"}} NM_032790 ; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..

    Sequencing:

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 (28) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence (NM_032790; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 ( 28 ) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_032790","term_id":"170932551","term_text":"NM_032790"}} NM_032790 ; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..

    Generated:

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 (28) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence (NM_032790; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters
    Article Snippet: The protein A-TEV protease-CBP tag (ATC)-TAP vector was made by subcloning the Kozak-PrA-TEV-CBP sequence [PCR-amplified from pBS1479 ( 28 ) into pcDNA4TO; Invitrogen]. .. In-frame subcloning of the human Orai1 coding sequence ( {"type":"entrez-nucleotide","attrs":{"text":"NM_032790","term_id":"170932551","term_text":"NM_032790"}} NM_032790 ; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA. ..



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    orai1 (nm_032790) human tagged orf clone  (OriGene)
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    Fig. 1. POST binds <t>Orai1</t> and is expressed in the ER and plasma membrane. (A) HA epitope-tagged POST specifically binds myc-tagged Orai1 coexpressed in HEK 293 cells. Cell lysates were immunoprecipitated with HA-agarose and stained on Western blot (WB) with myc- or HA-antibody conjugated to HRP. (B) Endogenous POST binds Orai1. Jurkat cell lysates were immunoprecipitated with Orai1, POST antibody, or preimmune rabbit IgG, and probed on WB with the indicated antibody (TrueBlot). Lysates of HEK 293 expressing myc-Orai1 (labeled as myc-Or1) and HA-POST were used as markers. Note that store depletion (cells treated with 1 μM thapsigargin for 10 min in Ca2+-free Ringer’s solution) did not affect POST-Orai1 interaction. (C) Live confocal fluorescence images of HEK 293 cells coexpressing POST-GFP and Cherry-STIM1. POST was localized in the cell periphery and intracellular membrane network, where it precisely colocalized with Cherry STIM1 (ER). Note the fraction of POST that is expressed in the cell periphery, where STIM1 is not expressed.
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    Image Search Results


    Fig. 1. POST binds Orai1 and is expressed in the ER and plasma membrane. (A) HA epitope-tagged POST specifically binds myc-tagged Orai1 coexpressed in HEK 293 cells. Cell lysates were immunoprecipitated with HA-agarose and stained on Western blot (WB) with myc- or HA-antibody conjugated to HRP. (B) Endogenous POST binds Orai1. Jurkat cell lysates were immunoprecipitated with Orai1, POST antibody, or preimmune rabbit IgG, and probed on WB with the indicated antibody (TrueBlot). Lysates of HEK 293 expressing myc-Orai1 (labeled as myc-Or1) and HA-POST were used as markers. Note that store depletion (cells treated with 1 μM thapsigargin for 10 min in Ca2+-free Ringer’s solution) did not affect POST-Orai1 interaction. (C) Live confocal fluorescence images of HEK 293 cells coexpressing POST-GFP and Cherry-STIM1. POST was localized in the cell periphery and intracellular membrane network, where it precisely colocalized with Cherry STIM1 (ER). Note the fraction of POST that is expressed in the cell periphery, where STIM1 is not expressed.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters.

    doi: 10.1073/pnas.1117231108

    Figure Lengend Snippet: Fig. 1. POST binds Orai1 and is expressed in the ER and plasma membrane. (A) HA epitope-tagged POST specifically binds myc-tagged Orai1 coexpressed in HEK 293 cells. Cell lysates were immunoprecipitated with HA-agarose and stained on Western blot (WB) with myc- or HA-antibody conjugated to HRP. (B) Endogenous POST binds Orai1. Jurkat cell lysates were immunoprecipitated with Orai1, POST antibody, or preimmune rabbit IgG, and probed on WB with the indicated antibody (TrueBlot). Lysates of HEK 293 expressing myc-Orai1 (labeled as myc-Or1) and HA-POST were used as markers. Note that store depletion (cells treated with 1 μM thapsigargin for 10 min in Ca2+-free Ringer’s solution) did not affect POST-Orai1 interaction. (C) Live confocal fluorescence images of HEK 293 cells coexpressing POST-GFP and Cherry-STIM1. POST was localized in the cell periphery and intracellular membrane network, where it precisely colocalized with Cherry STIM1 (ER). Note the fraction of POST that is expressed in the cell periphery, where STIM1 is not expressed.

    Article Snippet: In-frame subcloning of the human Orai1 coding sequence (NM_032790; Origene TC124465) into the ATC-TAP vector generated the N-terminal TAP-Orai1 cDNA.

    Techniques: Clinical Proteomics, Membrane, Immunoprecipitation, Staining, Western Blot, Expressing, Labeling